Infection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysis.

ABSTRACT:

Objectives

Swine have been regarded as intermediate hosts in the spread of influenza from birds to humans but studies of the sialylated glycans that comprise their respiratory tract have not been extensively studied in the past. This study analyzed the sialylated N-glycan and O-glycan profile of swine trachea and lung and correlated this with ex-vivo infection of swine explants with avian influenza viruses.

Sample

Lungs and tracheal samples were obtained from normal farm and laboratory raised swine and used for ex vivo infection as well as mass spectrometric analysis. Infection of the ex vivo tissues used high pathogenic and low pathogenic avian viruses including the novel H7N9 virus that emerged in China in early 2013.

Main outcome measures

Assessment of successful r

SUBMITTER: Chan RW 

PROVIDER: S-EPMC4114536 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature