In vivo detection of macrophage recruitment in hind-limb ischemia using a targeted near-infrared fluorophore.

ABSTRACT: Macrophages are an essential component of the immune system and have protective and pathogenic functions in various diseases. Imaging of macrophages in vivo could furnish new tools to advance evaluation of disease and therapies. Critical limb ischemia is a disease in which macrophages have considerable pathogenic roles, and are potential targets for cell-based immunotherapy. We sought to develop a new near-infrared fluorescence (NIRF) imaging probe to target macrophages specifically in vivo in various pathological states, including hind-limb ischemia. We rapidly screened the photostable cyanine-based NIRF library against different blood cell lines. The identified monocyte/macrophage-selective hit was tested in vitro in live-cell labeling assay. Non-invasive NIRF imaging was performed with

SUBMITTER: Yoo JS 

PROVIDER: S-EPMC4114964 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature