The different roles of myosin IIA and myosin IIB in contraction of 3D collagen matrices by human fibroblasts.

ABSTRACT: Contraction of 3D collagen matrices by fibroblasts frequently is used as an in vitro model of wound closure. Different iterations of the model - all conventionally referred to as "contraction" - involve different morphological patterns. During floating matrix contraction, cells initially are round without stress fibers and subsequently undergo spreading. During stressed matrix contraction, cells initially are spread with stress fibers and subsequently undergo shortening. In the current studies, we used siRNA silencing of myosin IIA (MyoIIA) and myosin IIB (MyoIIB) to test the roles of myosin II isoforms in fibroblast interactions with 3D collagen matrices and collagen matrix contraction. We found that MyoIIA but not MyoIIB was required for cellular global inward contractile force, formatio

SUBMITTER: Liu Z 

PROVIDER: S-EPMC4115049 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature