Long-term in vitro culture of grape berries and its application to assess the effects of sugar supply on anthocyanin accumulation.

ABSTRACT: Grape berry development and ripening are under complex regulation by the nutrients, hormones, and environment cues sensed by the berry. However, the biochemical and molecular mechanisms underlying these types of regulation are poorly understood. A simplified but realistic model system that enables fruit growth conditions to be modulated easily will facilitate the deciphering of these mechanisms. Here, an in vitro culture system of intact detached grape berries was developed by coupling the production of greenhouse fruiting-cuttings and in vitro organ culture techniques. (13)C and (15)N labelling experiments showed that this system enables the intact detached berries actively to absorb and utilize carbon and nitrogen from the culture medium. It was further used to study the effects of sugar

SUBMITTER: Dai ZW 

PROVIDER: S-EPMC4115254 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature