Bacterial spore detection and analysis using hyperpolarized ¹²⁹Xe chemical exchange saturation transfer (Hyper-CEST) NMR.

ABSTRACT: Previously, we reported hyperpolarized ¹²⁹Xe chemical exchange saturation transfer (Hyper-CEST) NMR techniques for the ultrasensitive (i.e., 1 picomolar) detection of xenon host molecules known as cryptophane. Here, we demonstrate a more general role for Hyper-CEST NMR as a spectroscopic method for probing nanoporous structures, without the requirement for cryptophane or engineered xenon-binding sites. Hyper-CEST ¹²⁹Xe NMR spectroscopy was employed to detect Bacillus anthracis and Bacillus subtilis spores in solution, and interrogate the layers that comprise their structures. ¹²⁹Xe-spore samples were selectively irradiated with radiofrequency pulses; the depolarized ¹²⁹Xe returned to aqueous solution and depleted the ¹²⁹X

SUBMITTER: Bai Y 

PROVIDER: S-EPMC4115335 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature