Genome-Wide Mapping of the Distribution of CarD, RNAP σ^A, and RNAP β on the Mycobacterium smegmatis Chromosome using Chromatin Immunoprecipitation Sequencing.

ABSTRACT: CarD is an essential mycobacterial protein that binds the RNA polymerase (RNAP) and affects the transcriptional profile of Mycobacterium smegmatis and Mycobacterium tuberculosis (6). We predicted that CarD was directly regulating RNAP function but our prior experiments had not determined at what stage of transcription CarD was functioning and at which genes CarD interacted with the RNAP. To begin to address these open questions, we performed Chromatin Immunoprecipitation sequencing (ChIP-seq) to survey the distribution of CarD throughout the M. smegmatis chromosome. The distribution of RNAP subunits β and σ^A were also profiled. We expected that RNAP β would be present throughout transcribed regions and RNAP σ^A would be predominantly enriched at pr

SUBMITTER: Landick R 

PROVIDER: S-EPMC4115788 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature