Influence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route.

ABSTRACT: The two-component regulatory system PhoP/PhoQ has been shown to (i) control expression of virulence-associated traits, (ii) confer survival and growth within macrophages and (iii) play a role in Yersinia infections. However, the influence of PhoP on virulence varied greatly between different murine models of infection and its role in natural oral infections with frequently used representative isolates of Y. pseudotuberculosis was unknown. To address this issue, we constructed an isogenic set of phoP+ and phoP- variants of strain IP32953 and YPIII and analyzed the impact of PhoP using in vitro functionality experiments and a murine oral infection model, whereby we tested for bacterial dissemination and influence on the host immune response. Our results revealed that PhoP has a low impact on

SUBMITTER: Pisano F 

PROVIDER: S-EPMC4116203 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature