Differences in heterocycle basicity distinguish homocysteine from cysteine using aldehyde-bearing fluorophores.

ABSTRACT: We report the detection of homocysteine over cysteine based upon characteristic differences between 5- and 6-membered heterocyclic amines formed upon reaction with aldehyde-bearing compounds. Homocysteine-derived thiazinane-4-carboxylic acids are more basic than cysteine-derived thiazolidines-4-carboxylic acids. Fluorescence enhancement in response to homocysteine is achieved by tuning pH and excitation wavelength.

SUBMITTER: Barve A 

PROVIDER: S-EPMC4116490 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature