Integrated analyses of proteins and their glycans in a magnetic bead-based multiplex assay format.

ABSTRACT:

Background

Well-annotated clinical samples are valuable resources for biomarker discovery and validation. Multiplex and integrated methods that simultaneously measure multiple analytes and generate integrated information about these analytes from a single measurement are desirable because these methods help conserve precious samples. We developed a magnetic bead-based system for multiplex and integrated glycoprotein quantification by immunoassays and glycan detection by lectin immunosorbent assays (LISAs).

Methods

Magnetic beads coupled with antibodies were used for capturing proteins of interest. Biotinylated antibodies in combination with streptavidin-labeled phycoerythrin were used for protein quantification. In the LISAs, biotinylated detection antibodies were replaced b

SUBMITTER: Li D 

PROVIDER: S-EPMC4116607 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature