High throughput automated chromatin immunoprecipitation as a platform for drug screening and antibody validation.

ABSTRACT: Chromatin immunoprecipitation (ChIP) is an assay for interrogating protein-DNA interactions that is increasingly being used for drug target discovery and screening applications. Currently the complexity of the protocol and the amount of hands-on time required for this assay limits its use to low throughput applications; furthermore, variability in antibody quality poses an additional obstacle in scaling up ChIP for large scale screening purposes. To address these challenges, we report HTChIP, an automated microfluidic-based platform for performing high-throughput ChIP screening measurements of 16 different targets simultaneously, with potential for further scale-up. From chromatin to analyzable PCR results only takes one day using HTChIP, as compared to several days up to one week for conv

SUBMITTER: Wu AR 

PROVIDER: S-EPMC4117344 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature