Identifying the lineages of individual cells in cocultures by multivariate analysis of Raman spectra.

ABSTRACT: The cellular and matrix cues that induce stem cell differentiation into distinct cell lineages must be identified to permit the ex vivo expansion of desired cell populations for clinical applications. Combinatorial biomaterials enable screening multiple different microenvironments while using small numbers of rare stem cells. New methods to identify the phenotypes of individual cells in cocultures with location specificity would increase the efficiency and throughput of these screening platforms. Here, we demonstrate that partial least-squares discriminant analysis (PLS-DA) models of calibration Raman spectra from cells in pure cultures can be used to identify the lineages of individual cells in more complex culture environments. The calibration Raman spectra were collected from individual

SUBMITTER: Ilin Y 

PROVIDER: S-EPMC4117405 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature