Hydrophobin film structure for HFBI and HFBII and mechanism for accelerated film formation.

ABSTRACT: Hydrophobins represent an important group of proteins from both a biological and nanotechnological standpoint. They are the means through which filamentous fungi affect their environment to promote growth, and their properties at interfaces have resulted in numerous applications. In our study we have combined protein docking, molecular dynamics simulation, and electron cryo-microscopy to gain atomistic level insight into the surface structure of films composed of two class II hydrophobins: HFBI and HFBII produced by Trichoderma reesei. Together our results suggest a unit cell composed of six proteins; however, our computational results suggest P6 symmetry, while our experimental results show P3 symmetry with a unit cell size of 56 Å. Our computational results indicate the possibility of an

SUBMITTER: Magarkar A 

PROVIDER: S-EPMC4117420 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature