Serial number tagging reveals a prominent sequence preference of retrotransposon integration.

ABSTRACT: Transposable elements (TE) have both negative and positive impact on the biology of their host. As a result, a balance is struck between the host and the TE that relies on directing integration to specific genome territories. The extraordinary capacity of DNA sequencing can create ultra dense maps of integration that are being used to study the mechanisms that position integration. Unfortunately, the great increase in the numbers of insertion sites detected comes with the cost of not knowing which positions are rare targets and which sustain high numbers of insertions. To address this problem we developed the serial number system, a TE tagging method that measures the frequency of integration at single nucleotide positions. We sequenced 1 million insertions of retrotransposon Tf1 in the ge

SUBMITTER: Chatterjee AG 

PROVIDER: S-EPMC4117765 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature