Efficient linkage mapping using exome capture and extreme QTL in schistosome parasites.

ABSTRACT:

Background

Identification of parasite genes that underlie traits such as drug resistance and host specificity is challenging using classical linkage mapping approaches. Extreme QTL (X-QTL) methods, originally developed by rodent malaria and yeast researchers, promise to increase the power and simplify logistics of linkage mapping in experimental crosses of schistosomes (or other helminth parasites), because many 1000s of progeny can be analysed, phenotyping is not required, and progeny pools rather than individuals are genotyped. We explored the utility of this method for mapping a drug resistance gene in the human parasitic fluke Schistosoma mansoni.

Results

We staged a genetic cross between oxamniquine sensitive and resistant parasites, then between two F1 progeny, to gene

SUBMITTER: Chevalier FD 

PROVIDER: S-EPMC4117968 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature