Galectin Hco-gal-m from Haemonchus contortus modulates goat monocytes and T cell function in different patterns.

ABSTRACT:

Background

Monocytes and T cells are two major subpopulations of peripheral blood mononuclear cells (PBMC) and play an essential role in the innate and adaptive immune systems. Different members of the galectin family show multiple and distinct regulatory effects on different cell types. Previous studies have demonstrated that the galectin from Haemonchus contortus (Hco-gal-m) performed immunomodulatory effects on goat PBMC, however, which subpopulation of PBMC is the primary target of Hco-gal-m and whether the immune modulations share the same mechanism remain unclear.

Methods

In this study, the developmental expression of Hco-gal-m was analyzed by RT-PCR and Western blot analysis. The distribution of Hco-gal-m in adult worm was detected by an immunohistochemical test. The

SUBMITTER: Wang W 

PROVIDER: S-EPMC4117971 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature