Genome-wide analysis of salt-responsive and novel microRNAs in Populus euphratica by deep sequencing.

ABSTRACT:

Background

Populus euphratica is a representative model woody plant species for studying resistance to abiotic stresses such as drought and salt. Salt stress is one of the most common environmental factors that affect plant growth and development. MicroRNAs (miRNAs) are small, noncoding RNAs that have important regulatory functions in plant growth, development, and response to abiotic stress.

Results

To investigate the miRNAs involved in the salt-stress response, we constructed four small cDNA libraries from P. euphratica plantlets treated with or without salt (300 mM NaCl, 3 days) in either the root or leaf. Using high-throughput sequencing to identify miRNAs, we found 164 conserved miRNAs belonging to 44 families. Of these, 136 novel miRNAs were from the leaf, and 128 nove

SUBMITTER: Si J 

PROVIDER: S-EPMC4118626 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature