Analysis of the leaf methylomes of parents and their hybrids provides new insight into hybrid vigor in Populus deltoides.

ABSTRACT:

Background

Plants with heterosis/hybrid vigor perform better than their parents in many traits. However, the biological mechanisms underlying heterosis remain unclear. To investigate the significance of DNA methylation to heterosis, a comprehensive analysis of whole-genome DNA methylome profiles of Populus deltoides cl.'55/65' and '10/17' parental lines and their intraspecific F1 hybrids lines was performed using methylated DNA immunoprecipitation (MeDIP) and high-throughput sequencing.

Results

Here, a total of 486.27 million reads were mapped to the reference genome of Populus trichocarpa, with an average unique mapping rate of 57.8%. The parents with similar genetic background had distinct DNA methylation levels. F1 hybrids with hybrid vigor possessed non-additive DNA meth

SUBMITTER: Gao M 

PROVIDER: S-EPMC4118634 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature