In silico single strand melting curve: a new approach to identify nucleic acid polymorphisms in Totiviridae.

ABSTRACT:

Background

The PCR technique and its variations have been increasingly used in the clinical laboratory and recent advances in this field generated new higher resolution techniques based on nucleic acid denaturation dynamics. The principle of these new molecular tools is based on the comparison of melting profiles, after denaturation of a DNA double strand. Until now, the secondary structure of single-stranded nucleic acids has not been exploited to develop identification systems based on PCR. To test the potential of single-strand RNA denaturation as a new alternative to detect specific nucleic acid variations, sequences from viruses of the Totiviridae family were compared using a new in silico melting curve approach. This family comprises double-stranded RNA virus, with a genome c

SUBMITTER: Oliveira RA 

PROVIDER: S-EPMC4119202 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature