Kinomic exploration of temozolomide and radiation resistance in Glioblastoma multiforme xenolines.

ABSTRACT:

Background and purpose

Glioblastoma multiforme (GBM) represents the most common and deadly primary brain malignancy, particularly due to temozolomide (TMZ) and radiation (RT) resistance. To better understand resistance mechanisms, we examined global kinase activity (kinomic profiling) in both treatment sensitive and resistant human GBM patient-derived xenografts (PDX or "xenolines").

Materials and methods

Thirteen orthotopically-implanted xenolines were examined including 8 with known RT sensitivity/resistance, while 5 TMZ resistant xenolines were generated through serial TMZ treatment in vivo. Tumors were harvested, prepared as total protein lysates, and kinomically analyzed on a PamStation®12 high-throughput microarray platform with subsequent upstream kinase prediction an

SUBMITTER: Anderson JC 

PROVIDER: S-EPMC4119546 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature