MiRSeq: a user-friendly standalone toolkit for sequencing quality evaluation and miRNA profiling.

ABSTRACT: MicroRNAs (miRNAs) present diverse regulatory functions in a wide range of biological activities. Studies on miRNA functions generally depend on determining miRNA expression profiles between libraries by using a next-generation sequencing (NGS) platform. Currently, several online web services are developed to provide small RNA NGS data analysis. However, the submission of large amounts of NGS data, conversion of data format, and limited availability of species bring problems. In this study, we developed miRSeq to provide alternatives. To test the performance, we had small RNA NGS data from four species, including human, rat, fly, and nematode, analyzed with miRSeq. The alignments results indicate that miRSeq can precisely evaluate the sequencing quality of samples regarding percentage of s

SUBMITTER: Pan CT 

PROVIDER: S-EPMC4119685 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature