Effects of rhinovirus species on viral replication and cytokine production.

ABSTRACT:

Background

Epidemiologic studies provide evidence of differential virulence of rhinovirus species (RV). We recently reported that RV-A and RV-C induced more severe illnesses than RV-B, which suggests that the biology of RV-B might be different from RV-A or RV-C.

Objective

To test the hypothesis that RV-B has lower replication and induces lesser cytokine responses than RV-A or RV-C.

Methods

We cloned full-length cDNA of RV-A16, A36, B52, B72, C2, C15, and C41 from clinical samples and grew clinical isolates of RV-A7 and RV-B6 in cultured cells. Sinus epithelial cells were differentiated at the air-liquid interface. We tested for differences in viral replication in epithelial cells after infection with purified viruses (10(8) RNA copies) and measured virus load by quant

SUBMITTER: Nakagome K 

PROVIDER: S-EPMC4119842 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature