Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B.

ABSTRACT:

Background

Tuberculosis is the leading cause of death due to bacterial infections worldwide, mainly caused by Mycobacterium tuberculosis. The antigen 85 complex comprises a set of major secreted proteins of M. tuberculosis, which are potential biomarkers for diagnostic.

Results

In this work, the first human single chain fragment variable (scFv) antibodies specific for the tuberculosis biomarker 85 B were selected by phage display from naïve antibody gene libraries (HAL7/8). Produced as scFv-Fc in mammalian cells, these antibodies were further characterized and analysed for specificity and applicability in different tuberculosis antigen detection assays. Sandwich detection of recombinant 85 B was successful in enzyme linked immunosorbent assay (ELISA), lateral flow immunoassa

SUBMITTER: Fuchs M 

PROVIDER: S-EPMC4119940 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature