Microfluidic platform to evaluate migration of cells from patients with DYT1 dystonia.

ABSTRACT:

Background

Microfluidic platforms for quantitative evaluation of cell biologic processes allow low cost and time efficient research studies of biological and pathological events, such as monitoring cell migration by real-time imaging. In healthy and disease states, cell migration is crucial in development and wound healing, as well as to maintain the body's homeostasis.

New method

The microfluidic chambers allow precise measurements to investigate whether fibroblasts carrying a mutation in the TOR1A gene, underlying the hereditary neurologic disease--DYT1 dystonia, have decreased migration properties when compared to control cells.

Results

We observed that fibroblasts from DYT1 patients showed abnormalities in basic features of cell migration, such as reduced velocity

SUBMITTER: Nery FC 

PROVIDER: S-EPMC4120252 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature