Mitogen-activated protein kinase phosphatase-1 promotes neovascularization and angiogenic gene expression.

ABSTRACT:

Objective

Angiogenesis is the formation of new blood vessels through endothelial cell sprouting. This process requires the mitogen-activated protein kinases, signaling molecules that are negatively regulated by the mitogen-activated protein kinase phosphatase-1 (MKP-1). The purpose of this study was to evaluate the role of MKP-1 in neovascularization in vivo and identify associated mechanisms in endothelial cells.

Approach and results

We used murine hindlimb ischemia as a model system to evaluate the role of MKP-1 in angiogenic growth, remodeling, and arteriogenesis in vivo. Genomic deletion of MKP-1 blunted angiogenesis in the distal hindlimb and microvascular arteriogenesis in the proximal hindlimb. In vitro, endothelial MKP-1 depletion/deletion abrogated vascular endothel

SUBMITTER: Boerckel JD 

PROVIDER: S-EPMC4121075 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature