A method for quantitative analysis of standard and high-throughput qPCR expression data based on input sample quantity.

ABSTRACT: Over the past decade rapid advances have occurred in the understanding of RNA expression and its regulation. Quantitative polymerase chain reactions (qPCR) have become the gold standard for quantifying gene expression. Microfluidic next generation, high throughput qPCR now permits the detection of transcript copy number in thousands of reactions simultaneously, dramatically increasing the sensitivity over standard qPCR. Here we present a gene expression analysis method applicable to both standard polymerase chain reactions (qPCR) and high throughput qPCR. This technique is adjusted to the input sample quantity (e.g., the number of cells) and is independent of control gene expression. It is efficiency-corrected and with the use of a universal reference sample (commercial complementary DNA (

SUBMITTER: Adamski MG 

PROVIDER: S-EPMC4121206 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature