Altered MCM protein levels and autophagic flux in aged and systemic sclerosis dermal fibroblasts.

ABSTRACT: Aging is a common risk factor of many disorders. With age, the level of insoluble extracellular matrix increases leading to increased stiffness of a number of tissues. Matrix accumulation can also be observed in fibrotic disorders, such as systemic sclerosis (SSc). Although the intrinsic aging process in skin is phenotypically distinct from SSc, here we demonstrate similar behavior of aged and SSc skin fibroblasts in culture. We have used quantitative proteomics to characterize the phenotype of dermal fibroblasts from healthy subjects of various ages and from patients with SSc. Our results demonstrate that proteins involved in DNA and RNA processing decrease with age and in SSc, whereas those involved in mitochondrial and other metabolic processes behave the opposite. Specifically, minichr

SUBMITTER: Dumit VI 

PROVIDER: S-EPMC4121389 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature