Repeatable, Inducible Micro-RNA-Based Technology Tightly Controls Liver Transgene Expression.

ABSTRACT: Inducible systems for gene expression emerge as a new class of artificial vectors offering temporal and spatial exogenous control of gene expression. However, most inducible systems are less efficient in vivo and lack the target-organ specificity. In the present study, we have developed and optimized an oligonucleotide-based inducible system for the in vivo control of transgenes in the liver. We generated a set of simple, inducible plasmid-vectors based on the addition of four units of liver-specific miR-122 target sites to the 3'untranslated region of the gene of interest. Once the vector was delivered into hepatocytes this modification induced a dramatic reduction of gene expression that could be restored by the infusion of an antagomir for miR-122. The efficiency of the system was teste

SUBMITTER: Oprea II 

PROVIDER: S-EPMC4121515 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature