Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1.

ABSTRACT: The directed movement of cells is critical for numerous developmental and disease processes. A developmentally reiterated form of migration is radial intercalation; the process by which cells move in a direction orthogonal to the plane of the tissue from an inner layer to an outer layer. We use the radial intercalation of cells into the skin of Xenopus laevis embryos as a model to study directed cell migration within an epithelial tissue. We identify a novel function for both the microtubule-binding protein CLAMP and members of the microtubule-regulating Par complex during intercalation. Specifically, we show that Par3 and aPKC promote the apical positioning of centrioles, whereas CLAMP stabilizes microtubules along the axis of migration. We propose a model in which the Par complex defines

SUBMITTER: Werner ME 

PROVIDER: S-EPMC4121976 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature