Interleukin 17-producing γδT cells promote hepatic regeneration in mice.

ABSTRACT:

Background & aims

Subsets of leukocytes synergize with regenerative growth factors to promote hepatic regeneration. γδT cells are early responders to inflammation-induced injury in a number of contexts. We investigated the role of γδT cells in hepatic regeneration using mice with disruptions in Tcrd (encodes the T-cell receptor δ chain) and Clec7a (encodes C-type lectin domain family 7 member a, also known as DECTIN1).

Methods

We performed partial hepatectomies on wild-type C57BL/6, CD45.1, Tcrd(-/-), or Clec7a(-/-) mice. Cells were isolated from livers of patients and mice via mechanical and enzymatic digestion. γδT cells were purified by fluorescence-activated cell sorting.

Results

In mice, partial hepatectomy up-regulated expression of CCL20 and ligands of Dectin-1

SUBMITTER: Rao R 

PROVIDER: S-EPMC4123443 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature