Automated long-term monitoring of parallel microfluidic operations applying a machine vision-assisted positioning method.

ABSTRACT: As microfluidics has been applied extensively in many cell and biochemical applications, monitoring the related processes is an important requirement. In this work, we design and fabricate a high-throughput microfluidic device which contains 32 microchambers to perform automated parallel microfluidic operations and monitoring on an automated stage of a microscope. Images are captured at multiple spots on the device during the operations for monitoring samples in microchambers in parallel; yet the device positions may vary at different time points throughout operations as the device moves back and forth on a motorized microscopic stage. Here, we report an image-based positioning strategy to realign the chamber position before every recording of microscopic image. We fabricate alignment mark

SUBMITTER: Yip HM 

PROVIDER: S-EPMC4124227 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature