A protein-RNA specificity code enables targeted activation of an endogenous human transcript.

ABSTRACT: Programmable protein scaffolds that target DNA are invaluable tools for genome engineering and designer control of transcription. RNA manipulation provides broad new opportunities for control, including changes in translation. PUF proteins are an attractive platform for that purpose because they bind specific single-stranded RNA sequences by using short repeated modules, each contributing three amino acids that contact an RNA base. Here, we identified the specificities of natural and designed combinations of those three amino acids, using a large randomized RNA library. The resulting specificity code reveals the RNA binding preferences of natural proteins and enables the design of new specificities. Using the code and a translational activation domain, we designed a protein that targets en

SUBMITTER: Campbell ZT 

PROVIDER: S-EPMC4125476 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature