Towards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.

ABSTRACT: Cryo-transmission electron microscopy (Cryo-TEM) and particularly single particle analysis is rapidly becoming the premier method for determining the three-dimensional structure of protein complexes, and viruses. In the last several years there have been dramatic technological improvements in Cryo-TEM, such as advancements in automation and use of improved detectors, as well as improved image processing techniques. While Cryo-TEM was once thought of as a low resolution structural technique, the method is currently capable of generating nearly atomic resolution structures on a routine basis. Moreover, the combination of Cryo-TEM and other methods such as X-ray crystallography, nuclear magnetic resonance spectroscopy, and molecular dynamics modeling are allowing researchers to address scient

SUBMITTER: Lengyel J 

PROVIDER: S-EPMC4125826 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature