Single-cell mass cytometry analysis of human tonsil T cell remodeling by varicella zoster virus.

ABSTRACT: Although pathogens must infect differentiated host cells that exhibit substantial diversity, documenting the consequences of infection against this heterogeneity is challenging. Single-cell mass cytometry permits deep profiling based on combinatorial expression of surface and intracellular proteins. We used this method to investigate varicella-zoster virus (VZV) infection of tonsil T cells, which mediate viral transport to skin. Our results indicate that VZV induces a continuum of changes regardless of basal phenotypic and functional T cell characteristics. Contrary to the premise that VZV selectively infects T cells with skin trafficking profiles, VZV infection altered T cell surface proteins to enhance or induce these properties. Zap70 and Akt signaling pathways that trigger such surface

SUBMITTER: Sen N 

PROVIDER: S-EPMC4127309 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature