Genome-wide analysis of miRNA-mRNA interactions in marrow stromal cells.

ABSTRACT: Regulation of hematopoietic stem cell proliferation, lineage commitment, and differentiation in adult vertebrates requires extrinsic signals provided by cells in the marrow microenvironment (ME) located within the bone marrow. Both secreted and cell-surface bound factors critical to this regulation have been identified, yet control of their expression by cells within the ME has not been addressed. Herein we hypothesize that microRNAs (miRNAs) contribute to their controlled expression. MiRNAs are small noncoding RNAs that bind to target mRNAs and downregulate gene expression by either initiating mRNA degradation or preventing peptide translation. Testing the role of miRNAs in downregulating gene expression has been difficult since conventional techniques used to define miRNA-mRNA interactio

SUBMITTER: Balakrishnan I 

PROVIDER: S-EPMC4127404 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature