Pair-wise comparison analysis of differential expression of mRNAs in early and advanced stage primary colorectal adenocarcinomas.

ABSTRACT:

Objectives

To characterise the mRNA expression patterns of early and advanced stage colorectal adenocarcinomas of Malaysian patients.

Design

Comparative expression analysis.

Setting and participants

We performed a combination of annealing control primer (ACP)-based PCR and reverse transcription-quantitative real-time PCR for the identification of differentially expressed genes (DEGs) associated with early and advanced stage primary colorectal tumours. We recruited four paired samples from patients with colorectal cancer (CRC) of Dukes' A and B for the preliminary differential expression study, and a total of 27 paired samples, ranging from CRC stages I to IV, for subsequent confirmatory test. The tumouric samples were obtained from the patients with CRC undergoing cur

SUBMITTER: Lau TP 

PROVIDER: S-EPMC4127931 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature