Establishment and characterization of a primary calcifying epithelial odontogenic tumor cell population.

ABSTRACT:

Unlabelled

Calcifying epithelial odontogenic tumors (CEOTs) are rare neoplasms derived from dental tissue with the unique characteristic of calcifying amyloid-like material.

Objectives

To establish primary CEOT epithelial-derived cell populations, investigate the expression of enamel matrix proteins (EMPs), and identify potential ameloblastin (AMBN) and patched 1 (PTCH1) gene alterations.

Materials and methods

A 28-year-old patient with a lesion of the posterior maxilla, radiographically characterized by a radiolucency with well-defined borders containing mixed radiopacities, agreed to participate with informed consent. The patient's biopsy confirmed the diagnosis of CEOT, and a small representative tumor fragment was ascertained for cell culture. Explant cultures wer

SUBMITTER: Amm HM 

PROVIDER: S-EPMC4128328 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature