Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells.

ABSTRACT:

Objective

To determine the molecular characteristics of human spermatogonia and optimize methods to enrich spermatogonial stem cells (SSCs).

Design

Laboratory study using human tissues.

Setting

Research institute.

Patient(s)

Healthy adult human testicular tissue.

Intervention(s)

Human testicular tissue was fixed or digested with enzymes to produce a cell suspension. Human testis cells were fractionated by fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS).

Main outcome measure(s)

Immunostaining for selected markers, human-to-nude mouse xenotransplantation assay.

Result(s)

Immunohistochemistry costaining revealed the relative expression patterns of SALL4, UTF1, ZBTB16, UCHL1, and ENO2 in human undifferentia

SUBMITTER: Valli H 

PROVIDER: S-EPMC4128386 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature