Noninvasive diagnosis of intraamniotic infection: proteomic biomarkers in vaginal fluid.

ABSTRACT:

Objective

We analyzed the vaginal fluid proteome to identify biomarkers of intraamniotic infection among women in preterm labor.

Study design

Proteome analysis was performed on vaginal fluid specimens from women with preterm labor, using multidimensional liquid chromatography, tandem mass spectrometry, and label-free quantification. Enzyme immunoassays were used to quantify candidate proteins. Classification accuracy for intraamniotic infection (positive amniotic fluid bacterial culture and/or interleukin-6 >2 ng/mL) was evaluated using receiver-operator characteristic curves obtained by logistic regression.

Results

Of 170 subjects, 30 (18%) had intraamniotic infection. Vaginal fluid proteome analysis revealed 338 unique proteins. Label-free quantification identified

SUBMITTER: Hitti J 

PROVIDER: S-EPMC4128483 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature