A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia.

ABSTRACT:

Background

We report a novel method of culturing microglia in three dimension (3D) using collagen as a substrate. By culturing microglia within a matrix, we aim to emulate the physical state of microglia embedded within parenchyma.

Methods

BV2 microglia cell suspensions were prepared with type I collagen and cast into culture plates. To characterise the BV2 microglia cultured in 3D, the cultures were evaluated for their viability, cell morphology and response to lipopolysaccharide (LPS) activation. Conventional monolayer cultures (grown on uncoated and collagen-coated polystyrene) were set up concurrently for comparison.

Results

BV2 microglia in 3D collagen matrices were viable at 48 hrs of culture and exhibit a ramified morphology with multiplanar cytoplasmic project

SUBMITTER: Haw RT 

PROVIDER: S-EPMC4128540 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature