Altered expression of diabetes-related genes in Alzheimer's disease brains: the Hisayama study.

ABSTRACT: Diabetes mellitus (DM) is considered to be a risk factor for dementia including Alzheimer's disease (AD). However, the molecular mechanism underlying this risk is not well understood. We examined gene expression profiles in postmortem human brains donated for the Hisayama study. Three-way analysis of variance of microarray data from frontal cortex, temporal cortex, and hippocampus was performed with the presence/absence of AD and vascular dementia, and sex, as factors. Comparative analyses of expression changes in the brains of AD patients and a mouse model of AD were also performed. Relevant changes in gene expression identified by microarray analysis were validated by quantitative real-time reverse-transcription polymerase chain reaction and western blotting. The hippocampi of AD brains

SUBMITTER: Hokama M 

PROVIDER: S-EPMC4128707 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature