Chemical and biochemical approaches in the study of histone methylation and demethylation.

ABSTRACT: Histone methylation represents one of the most critical epigenetic events in DNA function regulation in eukaryotic organisms. Classic molecular biology and genetics tools provide significant knowledge about mechanisms and physiological roles of histone methyltransferases and demethylases in various cellular processes. In addition to this stream line, development and application of chemistry and chemistry-related techniques are increasingly involved in biological study, and offer information otherwise difficult to obtain by standard molecular biology methods. Herein, we review recent achievements and progress in developing and applying chemical and biochemical approaches in the study of histone methylation, including chromatin immunoprecipitation, chemical ligation, mass spectrometry, bioch

SUBMITTER: Li KK 

PROVIDER: S-EPMC4129395 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature