α4 integrin is a regulator of leukocyte recruitment after experimental intracerebral hemorrhage.

ABSTRACT:

Background and purpose

Intracerebral hemorrhage (ICH) is swiftly followed by an inflammatory response. A key component of this response is the recruitment of leukocytes into the brain, which promotes neurological injury in rodent models. However, the mechanisms by which leukocytes transmigrate across the endothelium into the injured brain are unclear. The present study examines leukocyte adhesion molecules (α4 integrin, L-selectin, and αLβ2 integrin) on 4 leukocyte subtypes to determine which are important for leukocyte recruitment after ICH.

Methods

We used the blood injection mouse model of ICH, whereby 25 μL of blood was injected into the striatum. Flow cytometry was used to quantify leukocyte populations and adhesion molecule expression in brain and blood. An α4 integrin

SUBMITTER: Hammond MD 

PROVIDER: S-EPMC4129460 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature