Biosensor architectures for high-fidelity reporting of cellular signaling.

ABSTRACT: Understanding mechanisms of information processing in cellular signaling networks requires quantitative measurements of protein activities in living cells. Biosensors are molecular probes that have been developed to directly track the activity of specific signaling proteins and their use is revolutionizing our understanding of signal transduction. The use of biosensors relies on the assumption that their activity is linearly proportional to the activity of the signaling protein they have been engineered to track. We use mechanistic mathematical models of common biosensor architectures (single-chain FRET-based biosensors), which include both intramolecular and intermolecular reactions, to study the validity of the linearity assumption. As a result of the classic mechanism of zero-order ultr

SUBMITTER: Dushek O 

PROVIDER: S-EPMC4129486 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature