Classification of dynamical diffusion states in single molecule tracking microscopy.

ABSTRACT: Single molecule tracking of membrane proteins by fluorescence microscopy is a promising method to investigate dynamic processes in live cells. Translating the trajectories of proteins to biological implications, such as protein interactions, requires the classification of protein motion within the trajectories. Spatial information of protein motion may reveal where the protein interacts with cellular structures, because binding of proteins to such structures often alters their diffusion speed. For dynamic diffusion systems, we provide an analytical framework to determine in which diffusion state a molecule is residing during the course of its trajectory. We compare different methods for the quantification of motion to utilize this framework for the classification of two diffusion states (t

SUBMITTER: Bosch PJ 

PROVIDER: S-EPMC4129487 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature