Accelerated chromatin biochemistry using DNA-barcoded nucleosome libraries.

ABSTRACT: Elucidating the molecular details of how chromatin-associated factors deposit, remove and recognize histone post-translational modification (PTM) signatures remains a daunting task in the epigenetics field. We introduce a versatile platform that greatly accelerates biochemical investigations into chromatin recognition and signaling. This technology is based on the streamlined semisynthesis of DNA-barcoded nucleosome libraries with distinct combinations of PTMs. Chromatin immunoprecipitation of these libraries, once they have been treated with purified chromatin effectors or the combined chromatin recognizing and modifying activities of the nuclear proteome, is followed by multiplexed DNA-barcode sequencing. This ultrasensitive workflow allowed us to collect thousands of biochemical data po

SUBMITTER: Nguyen UT 

PROVIDER: S-EPMC4130351 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature