A mammaglobin-A targeting agent for noninvasive detection of breast cancer metastasis in lymph nodes.

ABSTRACT: Pathologic axillary lymph node (ALN) status is an important prognostic factor for staging breast cancer. Currently, status is determined by histopathology following surgical excision of sentinel lymph node(s), which is an invasive, time consuming, and costly procedure with potential morbidity to the patient. Here, we describe an imaging platform for noninvasive assessment of ALN status, eliminating the need for surgical examination of patients to rule out nodal involvement. A targeted imaging probe (MamAb-680) was developed by conjugation of a mammaglobin-A-specific monoclonal antibody to a near-infrared fluorescent dye. Using DNA and tissue microarray, mammaglobin-A was validated as a cell-surface target that is expressed in ALN-positive patient samples but is not expressed in normal lymp

SUBMITTER: Tafreshi NK 

PROVIDER: S-EPMC4130564 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature