MiR-20a inhibits cutaneous squamous cell carcinoma metastasis and proliferation by directly targeting LIMK1.

ABSTRACT:

Background

MicroRNA-20a (miR-20a) plays a key role in tumorigenesis and progression. But its function is reverse in different kinds of malignant tumor, and its role and mechanism in cutaneous squamous cell carcinoma (CSCC) remains unclear.

Object

To determine the miR-20a's roles in CSCC and confirm whether LIMK1 is a direct target gene of miR-20a.

Methods

First miR-20a and LIMK1 expression levels were detected in six pairs of CSCC tissues and corresponding normal skin by qRT-PCR. Then MTT assays and colony formation assays were performed to evaluate the impact of miR-20a on cell proliferation. In addition, scratch migration assays and transwell invasion assays were performed to check miR-20a's effect on cell metastasis. Since LIMK1 (LIM kinase-1) was predicted as a ta

SUBMITTER: Zhou J 

PROVIDER: S-EPMC4130727 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature