Osteopontin knockdown suppresses the growth and angiogenesis of colon cancer cells.

ABSTRACT:

Aim

To investigate the effects of osteopontin (OPN) gene expression knockdown on colon cancer Lovo cells in vitro.

Methods

Four candidate small interfering RNA (siRNA) constructs targeting the OPN gene and a scrambled control sequence (NC-siRNA) were synthesized and inserted into a pGPU6/GFP/Neo expression vector. After confirmation by restriction enzyme digestion and DNA sequencing, the recombinant plasmids were subsequently transfected into a human colon cancer cell line (Lovo) using a liposome transfection method. Stably transfected cells were maintained with G418 selection and referred to as Lovo-OPN-1, -2, -3, -4, and Lovo-NC cells. Knockdown efficiency of each of the four siRNA constructs was determined by real-time reverse transcription polymerase chain reaction assay

SUBMITTER: Wu XL 

PROVIDER: S-EPMC4130851 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature