Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles.

ABSTRACT:

Purpose

To assess the age-associated changes in oocytes and granulosa cells derived from early antral follicles (EAFs).

Method

Gene expression analysis of granulosa cells of the EAFs using a genome analyzer (Illumina) and in vitro culture of oocyte-granulosa cell complexes (OGCs) of EAFs (400-700 μm in diameter) collected from ovaries of aged (>120 months) and young (<50 months) cows.

Results

Gene expression profiles in granulosa cells of EAFs of aged cows, which included changes in genes that encode chaperone proteins and antioxidants. In vivo development of EAFs, as determined by oocyte diameter of EAFs and AFs (3-6 mm in diameter), appeared to be impaired in aged cows and the OGCs of aged cows contained low GSH compared to younger counterparts. When the OGCs were c

SUBMITTER: Itami N 

PROVIDER: S-EPMC4130926 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature