In depth annotation of the Anopheles gambiae mosquito midgut transcriptome.

ABSTRACT:

Background

Genome sequencing of Anopheles gambiae was completed more than ten years ago and has accelerated research on malaria transmission. However, annotation needs to be refined and verified experimentally, as most predicted transcripts have been identified by comparative analysis with genomes from other species. The mosquito midgut-the first organ to interact with Plasmodium parasites-mounts effective antiplasmodial responses that limit parasite survival and disease transmission. High-throughput Illumina sequencing of the midgut transcriptome was used to identify new genes and transcripts, contributing to the refinement of An. gambiae genome annotation.

Results

We sequenced ~223 million reads from An. gambiae midgut cDNA libraries generated from susceptible (G3) and ref

SUBMITTER: Padron A 

PROVIDER: S-EPMC4131051 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature